Del Mar Photonics - Femtosecond Lasers and their applications

Featured application:

Nanoscale spatial induction of ultraviolet photoproducts in cellular DNA by three-photon near-infrared absorption

Rosalind A Meldrum1, 3, Stanley W Botchway2, 4, Christopher W Wharton1 & Graeme J Hirst2
1 School of Biosciences, University of Birmingham, Birmingham, UK
2 Central Laser Facility, Central Laboratory of the Research Council, Rutherford Appleton Laboratory, Oxford, UK
3 School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
4 Central Laser Facility, CLRC, Rutherford Appleton Laboratory, Chilton, Didcot, Oxford OX11 0QX, UK


The high-resolution spatial induction of ultraviolet (UV) photoproducts in mammalian cellular DNA is a goal of many scientists who study UV damage and repair. Here we describe how UV photoproducts can be induced in cellular DNA within nanometre dimensions by near-diffraction-limited 750 nm infrared laser radiation. The use of multiphoton excitation to induce highly localized DNA damage in an individual cell nucleus or mitochondrion will provide much greater resolution for studies of DNA repair dynamics and intracellular localization as well as intracellular signalling processes and cell–cell communication. The technique offers an advantage over the masking method for localized irradiation of cells, as the laser radiation can specifically target a single cell and subnuclear structures such as nucleoli, nuclear membranes or any structure that can be labelled and visualized by a fluorescent tag. It also increases the time resolution with which migration of DNA repair proteins to damage sites can be monitored. We define the characteristics of localized DNA damage induction by near-infrared radiation and suggest how it may be used for new biological investigations.

See alo:

Localised induction of UV damage in cellular DNA for the study of DNA repair
dynamics. Comparison of the methods using a focused near infra-red laser beam
and a focused 248nm UV beam

Del Mar Photonics offers industry's most extensive portfolio of ultrafast lasers
We offer femtosecond oscillators and amplifiers based based on Ti:Sapphire (Trestles, Teahupoo, Cortes), Cr:Forsterite (Mavericks, Jaws), Yb- Solid State (Tourmaline-SS), Er-doped fiber (Tamarack, Buccaneers) and Yb- doped fibers (Tourmaline series). Main specifications of the basic standard models are summarized in the table below:

Laser brand-name  Brief description  Output parameters  Sample specifications  Pdf brochures
 Trestles  Ti:Sapphire oscillator  wavelength tuning range
 710-950 nm (@6W pump);
 output power >600 mW
 (@6W pump, 800 nm)
 pulse duration <20 fs - 100 fs
 Trestles-100 without pump laser
 Ti:Sapphire oscillator having a tuning range of
 710-950 nm (@6W pump);
 output power >600 mW (@6W pump, 800 nm);
 beam quality TEMoo;
 polarization - linear horizontal;
 pulse duration <100 fs;
 repetition rate 80 MHz
 Trestles Ti:Sapphire laser
 Trestles Finesse Ti:Sapphire laser
 Pump lasers
 HPG DPSS green laser
 Finesse DPSS green laser
 Teahupoo Rider  Ti:Sapphire one-box
 Mavericks  Cr:Forsterite oscillator

 tuning range – 1230-1270nm
 output power – up to 1W
 pulse duration – 60 fs - 80 fs
 repetition rate – 120/76 MHz (fixed)
Mavericks 65P
 Femtosecond Cr:Forsterite laser system
 Ytterbium pump laser included.
 tuning range – 1230-1270nm
 output power – 250 mW
 beam quality – TEMoo
 polarization – linear horizontal
 pulse duration – <65 fs
 repetition rate – 120/76 MHz (fixed)
 Electronic starter included.
 Peltier crystal head with temperature
 controller included.
Mavericks Cr:Forsterite femtosecond lasers

Multiphoton Imaging with Mavericks - introductory brochure

New compact version of Mavericks CrF:65P chromium forsterite femtosecond laser


 Tourmaline Yb-SS400  Ytterbium-doped Solid-
 State Femtosecond
 Wavelength, nm 1059±2
 Pulse duration fs < 150
 Output power, mW* >400
 Repetition rate, MHz 80*
 Tourmaline Yb-SS400/150
 Pulse duration (FWHM), fs < 150
 Wavelength, nm 1059±2
 Output power, mW* >400
 Repetition rate, MHz 80*
 Output power stability** ± 1% (see graph)
 Spatial mode TEMoo
 Polarization, linear >100:1 (horizontal)
 Laser head dimensions, mm 710x165x110
 Power supply dimensions, mm 230x200x85
 Ytterbium-doped Solid- State
 Femtosecond Oscillator
 Tamarack  Er-doped fiber
 femtosecond oscillator
 Wavelength, nm 1560±10
 Pulse Width, fs <80 - 250
 Output power, mW >10
 Tamarack 80 (RFQ)
 Pulse Width (FWHM), fs <80
 Wavelength, nm 1560±10
 Average output power, mW >10
 Peak output power, kW up to 1
 Repetition rate, MHz 70
 Power output:
 10 mW, TEM00, linearly polarized or fiber output
 Laser head dimensions, mm 180x210x50
 Power supply unit dimensions, mm 230x200x85
 Tamarack Er-doped femtosecond
 fiber laser
 Buccaneer  Er-doped fiber

 Buccaneer 100
 Femtosecond fiber-based laser system
 Wavelength (fixed) 1560±10nm; output
 power >100 mW;
 beam quality TEM00
 pulse duration (fixed) 150-300 fs;
 repetition rate (fixed) 60-80 MHz

 Buccaneer Er-doped
 femtosecond fiber laser
 Buccaneer SHG  Er-doped fiber
 oscillator/amplifier +SHG
 Wavelength 780+-10 nm
 Pulse Width < 120 fs
 Output power >40 mW
 Buccaneer with a SHG unit
 Fiber laser with SH generation
 Pulse Width (FWHM) < 120 fs
 Wavelength 780+-10 nm (fixed)
 Average output power >40 mW
 Repetition rate 40 MHz
 Spectral width ~ 7.5 nm
 RF Out: SMA
 Mode lock status: SMA
 Polarization: vertical
 Buccaneer Er-doped
 femtosecond fiber laser with build
 in SHG unit


 Tourmaline  Femtosecond Yb-based
 seed oscillator
 Output power 20mW
 Wavelength 1040-1064 nm
 Pulse duration 100 fs
 Tourmaline 100
 Average power 20mW
 Wavelength 1040-1064 nm
 Repetition rate 60-80 MHz
 Pulse duration 100 fs
 Output pulse energy 0.25nJ
 Femtosecond Yb-based seed
 Tourmaline 300-1  Femtosecond Yb-based
 laser system
 Pulse duration 300 fs
 Average power 1 W
 Tourmaline 300-1
Yb-based laser system
 Pulse duration 300 fs
 Average power 1 W
 Repetition rate 10 kHz
 Pulse energy 100 mkJ


 Tourmaline Yb-
 Yb-based high-energy fiber
 laser development kit,
 model Tourmaline Yb-
 Wavelength: 1.07 nm ±0.1 nm
 Pulse duration: 500 fs - 3 ns
 Pulse energy: up to 10 µJ
 Tourmaline Yb-ULRepRate-07
 Yb-based high-energy fiber laser system kit includes:
 - low-repetition-rate high-energy mode-locked Yb-
 doped all-fiber laser with open architecture which
 provides possibility of educational & experimental
 work for obtaining ultra-low-repetition-rate (up to 10
 kHz) and study of parabolic shaped pulses

 Wavelength: 1.07 nm ±0.1 nm
 Pulse duration: 500 fs - 3 ns
 Repetition rate: 25-5 MHz and low (up to 10 kHz)
 Pulse energy: up to 10 µJ (microJoule)


Del Mar Photonics, Inc.
4119 Twilight Ridge
San Diego, CA 92130
tel: (858) 876-3133
fax: (858) 630-2376