Del Mar Photonics - Femtosecond Lasers and their applications
Featured application:
Nanoscale spatial induction of ultraviolet photoproducts in cellular DNA by
three-photon near-infrared absorption
Rosalind A Meldrum1, 3, Stanley W Botchway2, 4, Christopher W Wharton1 & Graeme
J Hirst2
1 School of Biosciences, University of Birmingham, Birmingham, UK
2 Central Laser Facility, Central Laboratory of the Research Council, Rutherford
Appleton Laboratory, Oxford, UK
3 School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15
2TT, UK
4 Central Laser Facility, CLRC, Rutherford Appleton Laboratory, Chilton, Didcot,
Oxford OX11 0QX, UK
Abstract
The high-resolution spatial induction of ultraviolet (UV) photoproducts in
mammalian cellular DNA is a goal of many scientists who study UV damage and
repair. Here we describe how UV photoproducts can be induced in cellular DNA
within nanometre dimensions by near-diffraction-limited 750 nm infrared laser
radiation. The use of multiphoton excitation to induce highly localized DNA
damage in an individual cell nucleus or mitochondrion will provide much greater
resolution for studies of DNA repair dynamics and intracellular localization as
well as intracellular signalling processes and cell–cell communication. The
technique offers an advantage over the masking method for localized irradiation
of cells, as the laser radiation can specifically target a single cell and
subnuclear structures such as nucleoli, nuclear membranes or any structure that
can be labelled and visualized by a fluorescent tag. It also increases the time
resolution with which migration of DNA repair proteins to damage sites can be
monitored. We define the characteristics of localized DNA damage induction by
near-infrared radiation and suggest how it may be used for new biological
investigations.
See alo:
Del Mar Photonics offers industry's most extensive portfolio of ultrafast
lasers
We offer femtosecond oscillators and amplifiers based based on Ti:Sapphire
(Trestles, Teahupoo, Cortes), Cr:Forsterite (Mavericks, Jaws), Yb- Solid State
(Tourmaline-SS), Er-doped fiber
(Tamarack, Buccaneers) and Yb- doped fibers (Tourmaline series). Main
specifications of the basic standard models are summarized in the table below:
| Laser brand-name | Brief description | Output parameters | Sample specifications | Pdf brochures |
| Trestles | Ti:Sapphire oscillator | wavelength tuning range 710-950 nm (@6W pump); output power >600 mW (@6W pump, 800 nm) pulse duration <20 fs - 100 fs |
Trestles-100 without pump laser Ti:Sapphire oscillator having a tuning range of 710-950 nm (@6W pump); output power >600 mW (@6W pump, 800 nm); beam quality TEMoo; polarization - linear horizontal; pulse duration <100 fs; repetition rate 80 MHz |
Trestles
Ti:Sapphire laser Trestles Finesse Ti:Sapphire laser Manual Pump lasers HPG DPSS green laser Finesse DPSS green laser |
| Teahupoo Rider | Ti:Sapphire one-box Oscillator/Amplifier |
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| Mavericks | Cr:Forsterite oscillator | tuning range – 1230-1270nm output power – up to 1W pulse duration – 60 fs - 80 fs repetition rate – 120/76 MHz (fixed) |
Mavericks 65P Femtosecond Cr:Forsterite laser system Ytterbium pump laser included. tuning range – 1230-1270nm output power – 250 mW beam quality – TEMoo polarization – linear horizontal pulse duration – <65 fs repetition rate – 120/76 MHz (fixed) Electronic starter included. Peltier crystal head with temperature controller included. |
Mavericks Cr:Forsterite femtosecond lasers Multiphoton Imaging with Mavericks - introductory brochure New compact version of Mavericks CrF:65P chromium forsterite femtosecond laser
|
| Tourmaline Yb-SS400 | Ytterbium-doped Solid- State Femtosecond Oscillator |
Wavelength, nm 1059±2 Pulse duration fs < 150 Output power, mW* >400 Repetition rate, MHz 80* |
Tourmaline Yb-SS400/150 Pulse duration (FWHM), fs < 150 Wavelength, nm 1059±2 Output power, mW* >400 Repetition rate, MHz 80* Output power stability** ± 1% (see graph) Spatial mode TEMoo Polarization, linear >100:1 (horizontal) Laser head dimensions, mm 710x165x110 Power supply dimensions, mm 230x200x85 |
Ytterbium-doped Solid- State
Femtosecond Oscillator |
| Tamarack | Er-doped
fiber femtosecond oscillator |
Wavelength, nm 1560±10 Pulse Width, fs <80 - 250 Output power, mW >10 |
Tamarack 80 (RFQ) Pulse Width (FWHM), fs <80 Wavelength, nm 1560±10 Average output power, mW >10 Peak output power, kW up to 1 Repetition rate, MHz 70 Power output: 10 mW, TEM00, linearly polarized or fiber output (FC/APC) Laser head dimensions, mm 180x210x50 Power supply unit dimensions, mm 230x200x85 |
Tamarack
Er-doped femtosecond fiber laser |
| Buccaneer | Er-doped
fiber femtosecond oscillator/amplifier |
Buccaneer 100 |
Buccaneer
Er-doped femtosecond fiber laser |
|
| Buccaneer SHG | Er-doped
fiber femtosecond oscillator/amplifier +SHG |
Wavelength 780+-10 nm Pulse Width < 120 fs Output power >40 mW |
Buccaneer with a SHG unit Fiber laser with SH generation Pulse Width (FWHM) < 120 fs Wavelength 780+-10 nm (fixed) Average output power >40 mW Repetition rate 40 MHz Spectral width ~ 7.5 nm RF Out: SMA Mode lock status: SMA Polarization: vertical |
Buccaneer
Er-doped femtosecond fiber laser with build in SHG unit |
| Tourmaline | Femtosecond Yb-based seed oscillator |
Output power 20mW Wavelength 1040-1064 nm Pulse duration 100 fs |
Tourmaline 100 Average power 20mW Wavelength 1040-1064 nm Repetition rate 60-80 MHz Pulse duration 100 fs Output pulse energy 0.25nJ |
Femtosecond Yb-based
seed oscillator |
| Tourmaline 300-1 | Femtosecond Yb-based laser system |
Pulse duration 300 fs Average power 1 W |
Tourmaline 300-1 Yb-based laser system Pulse duration 300 fs Average power 1 W Repetition rate 10 kHz Pulse energy 100 mkJ
|
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| Tourmaline Yb- ULRepRate-07 |
Yb-based
high-energy fiber laser development kit, model Tourmaline Yb- ULRepRate-07 |
Wavelength: 1.07 nm ±0.1 nm Pulse duration: 500 fs - 3 ns Pulse energy: up to 10 µJ |
Tourmaline Yb-ULRepRate-07 Yb-based high-energy fiber laser system kit includes: - low-repetition-rate high-energy mode-locked Yb- doped all-fiber laser with open architecture which provides possibility of educational & experimental work for obtaining ultra-low-repetition-rate (up to 10 kHz) and study of parabolic shaped pulses Wavelength: 1.07 nm ±0.1
nm |
Del Mar Photonics, Inc.
4119 Twilight Ridge
San Diego, CA 92130
tel: (858) 876-3133
fax: (858) 630-2376
sales@dmphotonics.com